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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has been noticed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a crucial function in endorsing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two supplemental splice variants ended up described but specific nucleotide sequence information and facts is not nonetheless readily available.

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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide generally consists of the epitope identified via the antibody.

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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...

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This manual outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...

Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is particular.

Focus on Info Cyclin E belongs on the remarkably conserved cyclin family, whose associates are characterized by a extraordinary periodicity in protein abundance in the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which contribute to your temporal coordination of every mitotic occasion. Cyclin E sorts a posh with and functions as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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